Diagnostic Immunology Topic Center

Serving as the expert scientific and educational resource for the College of American Pathologists (CAP).

Educational resources on diagnostic immunology and flow cytometry

Immunology-Related Journal Articles

• Criteria for Diagnosis, Staging, Risk Stratification and Response Assessment of Multiple Myeloma
• Screening and Diagnosis of Monoclonal Gamppathies. An International Survey of Laboratory Practice.

Flow Cytometry-Related Journal Articles

• CAP Flow Cytometry Checklist; Difficult and New Items (International Clinical Cytometry Society (ICCS) e-Newsletter, 2015:6(2)) (PDF, 1.4 MB)
• Marked Variability in Reported Minimal Residual Disease Lower Level of Detection of 4 Hematolymphoid Neoplasms
• EuroFlow Standardization of Flow Cytometer Instrument Settings and Immunophenotyping Protocols
• EuroFlow Antibody Panels for Standardized N-dimensional Flow Cytometric Immunophenotyping of Normal, Reactive and Malignant Leukocytes
• Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group (PDF, 655, KB)
• Diagnosing PNH with FLAER and multiparameter flow cytometry (PDF, 1.4 KB)
• Practice parameter for the diagnosis and management of primary immunodeficiency (PDF, 458 KB)
• Establishment and Validation of a Standard Protocol for the Detection of Minimal Residual Disease in B Lineage Childhood Acute Lymphoblastic Leukemia by Flow Cytometry in a Multi-Center Setting (PDF, 171 KB)
• Minimal Residual Disease Quantitation in Acute Myeloid Leukemia (PDF, 175 KB)
• Report of the European Myeloma Network on Multiparametric Flow Cytometry in Multiple Myeloma and Related Disorders (PDF, 192 KB)

Individualized Quality Control Plan Frequently Asked Questions

• IQCP FAQs

Guidelines, Consensus Statements, and Recommendations

• International Myeloma Working Group Guidelines for Serum-Free Light Chain Analysis in Multiple Myeloma and Related Disorders (PDF, 347 KB)
• NCCN guidelines on multiple myeloma (PDF, 301 KB)
• 2006 Bethesda International Consensus Recommendations
  • Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications (The Cytometry Part B: Clinical Cytometry Supplement 72B:S5-S13, 2007)
  • Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Optimal Reagents and Reporting for the Flow Cytometric Diagnosis of Hematopoietic Neoplasia (The Cytometry Part B: Clinical Cytometry Supplement 72B:S14-S22, 2007)
  • Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Recommendations for Training and Education to Perform Clinical Flow Cytometry (The Cytometry Part B: Clinical Cytometry Supplement 72B:S23-S33, 2007)
• The International Consensus Statement on an update of the Classification Criteria for Definite Antiphospholipid Syndrome [(APS) Miyakis S, Lockshin MD, Atsumi T, et al. JThromb Haemost. 2006;4:295-306)
• Consensus Guidelines on Anti-Beta 2 Glycoprotein I Testing and Reporting (Wong RCW, Favaloro EJ, Adelstein S, et al. Pathology. 2008;40:58-63)
• Update of the Guidelines for Lupus Anticoagulant Detection (Pengo V, Tripodi A, Reber G, et al. J Thromb Haemost. 2009;7:1737-1740)

Clinical and Laboratory Standards Institute Documents

• Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline - Second Edition (Clinical and Laboratory Standards Institute, document H43-A2)
• Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline - Second Edition (Clinical and Laboratory Standards Institute, document H42-A2)
• Evaluation Methods and Analytical Performance Characteristics of Immunological Assays for Human Immunoglobulin E (IgE) Antibodies of Defined Allergen Specificities; Approved Guideline (Clinical and Laboratory Standards Institute, document I/LA20-A)
• Quality Assurance of Laboratory Tests for Autoantibodies to Nuclear Antigens: (1) Indirect Fluorescence Assay for Microscopy and (2) Microtiter Enzyme Immunoassay Methods (Clinical and Laboratory Standards Institute, document ILA02-A2)

Join the Diagnostic Immunology and Flow Matrix Committee

Learn more about our committee activities and how to join the Diagnostic Immunology and Flow Matrix Committee.